Dr. Marta Princz
Liquid Chromatography - AKTA Systems (GE Healthcare Life Sciences)
Overview
AKTA Avant 150
- The ÄKTA Avant 150 is a preparative fast pressure liquid chromatography system designed for the development of scalable protein purification methods, as well as fine tuning of a purification method. Purification may be via gel filtration, affinity, and ion exchange, with sample detection with UV/Vis absorbance, conductivity and pH monitors. It houses an injection port and sample pump for sample loading of various volumes. Product integrity may be maintained with the internal temperature-controlled fraction collector, which allows fixed volume and peak fractionation. This system is operated by GE Unicorn 6™ control software, which features BufferPro, a buffer preparation function and Design of Experiments (DoE) to maximize output while minimizing time.
AKTAxpress
- The ÄKTAxpress is an automated fast pressure liquid chromatography system designed for multistep protein purification or tandem multiple protein purifications, where the purification methods have already been determined. It houses two buffer pumps and one sample pump, along with 5 superloops to store samples between columns. The entire unit is contained in a cold cabinet to maintain sample integrity. This system is operated by GE Unicorn 5.31™ control software.
Instrument Uses
- Compound Separation
- Protein Purification
Materials
- Liquids
Requirements
- Chromatography Column(s)
- Solutions 0.2 um filtered and degassed
- This instrument requires prior set-up by a BI Technician
